Quantitative RT-PCR Amplification of RNA in Single Mouse Oocytes and Preimplantation Embryos
نویسندگان
چکیده
منابع مشابه
Quantitative RT-PCR amplification of RNA in single mouse oocytes and preimplantation embryos.
We describe a simple whole-cell method for quantitative reverse transcription (RT) PCR amplification of RNA that consistently allows the analysis of trace amounts of RNA, such as those carried by a fraction of a single mouse oocyte or preimplantation embryo, without organic extraction. The method is based on a preliminary genomic DNA digestion by DNase I in the presence of Mn++ and a subsequent...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98244st02